Minimal Medium Rescue Screen

Screen paired minimal, complete, and supplemented-medium observations for a growth-rescue pattern; does not identify the deficient pathway.

Description

Growth rescue compares a poor minimal-medium response with complete and supplemented media.

Growth rescue compares a poor minimal-medium response with complete and supplemented media. A supplement-associated rescue pattern suggests a dependency under the observed conditions without identifying the deficient pathway.^[1]

Screen paired minimal, complete, and supplemented-medium observations for a growth-rescue pattern; does not identify the deficient pathway.

When to use Minimal Medium Rescue Screen

Use this tool to screen paired minimal, complete, and supplemented-medium observations for a growth-rescue pattern; does not identify the deficient pathway.

Prepare the sample, count, growth or assay measurements requested by this tool. Confirm units, sampling periods and reporting bases; supply independently established comparison criteria where requested.

How to use Minimal Medium Rescue Screen and understand its results

Prepare the sample, count, growth or assay measurements requested by this tool. Confirm units, sampling periods and reporting bases; supply independently established comparison criteria where requested.
Enter minimalGrowthOd, completeGrowthOd, supplementedGrowthOd, minimumGrowthOd using the field table and calculation rules, then run the tool.
Read minimalGrowthDetected, completeGrowthDetected, supplementRescuesGrowth, auxotrophyPattern. The explanation below defines the units, signs, ordering and any flags or placeholder values.
If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.

Input field	Meaning	Value type; see rules below	Requirement
minimalGrowthOd	Minimal growth od	number	Required
completeGrowthOd	Complete growth od	number	Required
supplementedGrowthOd	Supplemented growth od	number	Required
minimumGrowthOd	Minimum growth od	number	Required

Enter nonnegative OD observations and a positive growth threshold. Each growth test includes equality. supplementRescuesGrowth requires minimal below threshold and supplemented at/above it; auxotrophyPattern additionally requires complete-medium growth. Return these flags separately.

auxotrophyPattern = no minimal growth AND complete growth AND supplement rescue

Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.

Output field	Meaning	Value type
minimalGrowthDetected	Minimal growth detected	boolean
completeGrowthDetected	Complete growth detected	boolean
supplementRescuesGrowth	Supplement rescues growth	boolean
auxotrophyPattern	Auxotrophy pattern	boolean

json

{
  "input": {
    "minimalGrowthOd": 0.05,
    "completeGrowthOd": 0.5,
    "supplementedGrowthOd": 0.4,
    "minimumGrowthOd": 0.2
  },
  "output": {
    "minimalGrowthDetected": false,
    "completeGrowthDetected": true,
    "supplementRescuesGrowth": true,
    "auxotrophyPattern": true
  }
}

Limitations and assumptions

Blank correction, controls and comparability of assay times must be established independently. This pattern does not identify a mutation or pathway, and complete-medium failure can prevent the final flag even when supplemented growth is observed.

Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors. A successfully returned false flag is an evaluated outcome; its meaning is explained above.

Alternative or Complementary methods

Use Of Open Sealed Reaction: Decode paired open/sealed oxidative-fermentative observations while separating no-growth and discordant cases. For a complementary task, use Stationary Phase Window Audit: Screen the final growth-curve window for a caller-defined relative stability band, rather than equating one count with carrying capacity.

References

Minimal Medium Rescue Screen — tool.io

Similar or alternative tools

Of Open Sealed Reaction
Decode paired open/sealed oxidative-fermentative observations while separating no-growth and discordant cases.
Stationary Phase Window Audit
Screen the final growth-curve window for a caller-defined relative stability band, rather than equating one count with carrying capacity.