Motility Hanging Drop Motion Score Rater

Score independently tracked hanging-drop displacement against a caller motion threshold; Brownian-only or unknown observations are not positive motility.

Description

Score independently tracked hanging-drop displacement against a caller motion threshold; Brownian-only or unknown observations are not positive motility.

Directed displacement and Brownian movement are different kinds of motion evidence. A thresholded displacement rate can summarize a supplied observation without identifying a bacterium.

When to use this tool

Score independently tracked hanging-drop displacement against a caller motion threshold; Brownian-only or unknown observations are not positive motility.

  • Prepare the supplied netDisplacementMicrometres, elapsedSeconds, callerMotionThreshold, brownianOnly, observationKnown fields for this operation.
  • Compare the complete example with the representation used by your own input before carrying the result into another step.

How to use the tool and how it works

  1. Enter netDisplacementMicrometres, elapsedSeconds, callerMotionThreshold, brownianOnly, observationKnown. Use the exact text syntax, units and index conventions explained below.
  2. Run the tool. If input validation fails, correct the supplied fields before interpreting any result.
  3. Read result.observedSpeedMicrometresPerSecond, result.motionEvidence. Interpret false, null and empty lists using the distinctions below; they can be valid outputs.

Require nonnegative netDisplacementMicrometres/callerMotionThreshold and positive elapsedSeconds, then divide displacement by time. Label unknown if observationKnown is false; otherwise prefer brownian-only when brownianOnly is true, then directed-motion-supported only when speed is strictly above the threshold, or not-above-threshold otherwise.1

Input fieldMeaning and representationAccepted input
netDisplacementMicrometresnet displacement micrometres.Finite number; zero or greater
elapsedSecondselapsed seconds.Finite number; greater than zero
callerMotionThresholdcaller motion threshold.Finite number; zero or greater
brownianOnlyExplicit choice for brownian only; supply a boolean rather than a text label.true or false
observationKnownTrue only when the feature observation is actually known; false preserves an indeterminate/unknown outcome.true or false
Output fieldInterpretation
resultObject containing the named outputs below.
result.observedSpeedMicrometresPerSecondThe observed speed micrometres per second value under the stated formula or text convention.
result.motionEvidenceThe motion evidence value under the stated formula or text convention.
json
{
  "input": {
    "netDisplacementMicrometres": 4,
    "elapsedSeconds": 2,
    "callerMotionThreshold": 1,
    "brownianOnly": false,
    "observationKnown": true
  },
  "output": {
    "result": {
      "observedSpeedMicrometresPerSecond": 2,
      "motionEvidence": "directed-motion-supported"
    }
  }
}

Limitations and assumptions

  • The speed is still calculated when the observation is unknown or Brownian-only. No video tracking, drift correction or assay control is evaluated; equality with the supplied threshold does not pass and no species conclusion is drawn.
  • Every declared top-level field is required and unexpected fields are rejected. Numeric inputs must be finite; ordinary text fields are bounded to 8,192 UTF-16 code units, with tighter operation-specific limits where stated.
  • Ordinary string lists have at most 128 entries of at most 128 code units each; numeric lists and matrices have separate bounded size checks. Nonfinite results and excessively large serialized outputs are rejected.
  • Indexes are zero-based and spans have an exclusive end unless explicitly stated otherwise. A valid false, null or empty-list result is distinct from malformed input.

Alternative or Complementary methods

Retain specimen context, controls and independently observed features alongside a test label. Use a complete validated identification or analytical workflow when conclusions require several assays; a single stored morphology definition, feature flag or threshold comparison does not identify an organism.

References

  1. Tool behavior and example: Motility Hanging Drop Motion Score Rater — tool.io

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