Eclipse Latent Period Timeline
Separate observed eclipse, latent, and release-delay intervals from a one-step infection timeline.
Description
Calculate laboratory eclipse, latent, and release-delay durations from ordered adsorption, intracellular detection, and extracellular progeny times.
In a laboratory virus growth experiment, the eclipse interval concerns the appearance of detectable infectious progeny inside cells, while the latent interval extends to detectable extracellular progeny. These event-based timings describe different stages from adsorption through production and release.1
This tool uses adsorption completion as its reference event. It subtracts that coordinate from the first intracellular and extracellular detection times, then subtracts intracellular detection from extracellular detection to give a release delay. Reported durations therefore depend on the selected origin and assay events.
Eclipse Latent Period Timeline converts three supplied event hours into three durations. It does not locate those events in raw measurements, and its laboratory latent period is distinct from an epidemiological period before infectiousness.
When to summarize already identified experimental events
Use the tool after adsorption completion and both detection times have been determined for a comparable experiment. It helps keep event definitions and subtraction order explicit.
- Calculate all three delays on a shared hour axis.
- Check that the supplied event sequence is chronologically consistent.
- Distinguish adsorption-based durations from absolute observation hours.
How to enter event hours and calculate experimental delays
- Enter nonnegative Adsorption complete hour on the experiment’s hour axis.
- Enter First intracellular infectious hour and First extracellular progeny hour on that same axis.
- Ensure adsorption ≤ intracellular detection ≤ extracellular detection.
- Run the calculation and read Eclipse hours, Latent hours, and Release delay hours with the underlying event definitions.
eclipse = intracellularHour − adsorptionHour; latent = extracellularHour − adsorptionHour; releaseDelay = extracellularHour − intracellularHour The implementation checks the ordering and subtracts directly. With these definitions, latent hours equal eclipse hours plus release-delay hours.
{
"input": {
"adsorptionCompleteHour": 0,
"firstIntracellularInfectiousHour": 2,
"firstExtracellularProgenyHour": 3
},
"output": {
"eclipseHours": 2,
"latentHours": 3,
"releaseDelayHours": 1
}
}Equal event times are allowed and can give zero durations. Fractional hours are accepted. A shifted but otherwise identical hour origin preserves all durations when the nonnegative/order rules hold. Invalid ordering, a negative adsorption time, missing fields, or nonfinite inputs produce errors.
Limitations and interpretation
- The tool calculates delays from supplied observations rather than inferring exact continuous biological event times. Sampling and detection thresholds affect their meaning.
- No infection synchronization, assay quality, background correction, event interpolation, or uncertainty interval is assessed.
- Using another reference event changes reported eclipse and latent durations; preserve the adsorption-completion convention.
- Laboratory eclipse/latent terminology should not be substituted for population incubation or SEIR latent-duration parameters without a separate definition.
Alternative or Complementary methods
A sampled rise detector applies a supplied titer threshold to a time series. A SEIR transition calculator uses an epidemiological exposed compartment and a latency model; it is a different interpretation of latent timing.
References
-
The Growth of Bacteriophage — Journal of General Physiology; E. L. Ellis and M. Delbrück (1939)
Similar or alternative tools
- One Step Rise Onset
Locate the first sampled titer at or above a supplied baseline-fold rise.
- SEIR Latent Transition
Project exits from an epidemiological exposed compartment under exponential latency.