A260/230 Organic Contaminant Ratio Checker
Screen observed A260/A230 against an explicit caller ratio boundary; low ratio does not identify a specific organic contaminant.
Description
Screen observed A260/A230 against an explicit caller ratio boundary; low ratio does not identify a specific organic contaminant.
Absorbance ratios can be screened against a laboratory-chosen threshold before interpreting sample quality. A low ratio is a flag, not the identity of a contaminant.
When to use this tool
Screen observed A260/A230 against an explicit caller ratio boundary; low ratio does not identify a specific organic contaminant.
- Prepare the supplied a260, a230, callerMinimumRatio fields for this operation.
- Compare the complete example with the representation used by your own input before carrying the result into another step.
How to use the tool and how it works
- Enter a260, a230, callerMinimumRatio. Use the exact text syntax, units and index conventions explained below.
- Run the tool. If input validation fails, correct the supplied fields before interpreting any result.
- Read result.ratio, result.belowCallerMinimum, result.contaminantIdentified. Interpret false, null and empty lists using the distinctions below; they can be valid outputs.
Require nonnegative a260, positive a230 and a nonnegative callerMinimumRatio. Compute a260/a230, flag only when strictly below that minimum, and return contaminantIdentified false.1
| Input field | Meaning and representation | Accepted input |
|---|---|---|
| a260 | a260. | Finite number; zero or greater |
| a230 | a230. | Finite number; greater than zero |
| callerMinimumRatio | caller minimum ratio. | Finite number; zero or greater |
| Output field | Interpretation |
|---|---|
| result | Object containing the named outputs below. |
| result.ratio | The ratio value under the stated formula or text convention. |
| result.belowCallerMinimum | Flag for below caller minimum under the exact condition described above. |
| result.contaminantIdentified | Flag for contaminant identified under the exact condition described above. |
{
"input": {
"a260": 1,
"a230": 1,
"callerMinimumRatio": 2
},
"output": {
"result": {
"ratio": 1,
"belowCallerMinimum": true,
"contaminantIdentified": false
}
}
} Limitations and assumptions
- No contaminant is identified and no baseline or measurement uncertainty is modeled. The threshold is supplied by the caller; passing it does not establish purity or experimental suitability.
- Every declared top-level field is required and unexpected fields are rejected. Numeric inputs must be finite; ordinary text fields are bounded to 8,192 UTF-16 code units, with tighter operation-specific limits where stated.
- Ordinary string lists have at most 128 entries of at most 128 code units each; numeric lists and matrices have separate bounded size checks. Nonfinite results and excessively large serialized outputs are rejected.
- Indexes are zero-based and spans have an exclusive end unless explicitly stated otherwise. A valid false, null or empty-list result is distinct from malformed input.
Alternative or Complementary methods
Use a complete assay-analysis workflow for normalization, quality control and inference. Keep supplied measurements, fixed educational windows and caller-chosen thresholds distinct; these tools perform the documented calculation or retrieve a short reference note.
References
Similar or alternative tools
- A260/280 Purity Ratio 1.8-2.1 Window Checker
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