Hershberger Assay Calculator
actual Hershbergerandrogen-dependent tissue-weight comparison with independently identified species/sex, tissues and assay conditions. Does not infer hormonal mechanism, statistical effect significance or guideline-positive outcome; animal procedures outside scope.
Description
A Hershberger tissue-weight comparison uses independently identified tissues and matched body masses under a stated assay.
A Hershberger tissue-weight comparison uses independently identified tissues and matched body masses under a stated assay.1
Normalized mass and control fold change summarize observations without determining a hormonal mechanism.
When to use Hershberger Assay Calculator
Use this tool to calculate or review the supplied observations under the stated rules. Establish the chemical, exposure route, endpoint, measurement basis and independently applicable assay or assessment reference.
How to use Hershberger Assay Calculator and understand its results
- Establish the chemical, exposure route, endpoint, measurement basis and independently applicable assay or assessment reference.
- Enter actualIndependentlyMeasuredTissueMassesMg, matchedIndependentlyMeasuredBodyMassesGrams, callerVerifiedMatchedControlNormalizedMassMgPerGram, using the field meanings and rules below, then run the tool.
- Read result.observedBodyNormalizedTissueMassesMgPerGram, result.observedNormalizedMassFoldChanges. Use the units and subtraction order below to interpret each value; retain negative differences where returned.
- Correct any reported input issue and run again. Before using a successful result, check the stated units, signs, reference scope and observation coverage.
| Input field | Meaning | Value type | Requirement |
|---|---|---|---|
| actualIndependentlyMeasuredTissueMassesMg | Actual independently measured tissue masses mg | list of number | Required |
| matchedIndependentlyMeasuredBodyMassesGrams | Matched independently measured body masses grams | list of number | Required |
| callerVerifiedMatchedControlNormalizedMassMgPerGram | Caller verified matched control normalized mass mg per gram | number | Required |
Supply nonempty aligned nonnegative tissue masses in milligrams and strictly positive matched body masses in grams, plus a positive independently normalized control reference in milligrams per gram. Compute each tissue/body ratio, then divide each ratio by that control. Retain the individual results in input order.
normalizedMgPerG_i=tissueMassMg_i/bodyMassG_i; fold_i=normalizedMgPerG_i/controlNormalizedMgPerG Use the matching field units and the exact boundary, ordering and reference rules above.
| Output field | Meaning | Value type |
|---|---|---|
| result | Qualified observed calculation or explicit reference | object |
| result.observedBodyNormalizedTissueMassesMgPerGram | Individual tissue mass divided by matched body mass, in mg/g and input order. | list |
| result.observedNormalizedMassFoldChanges | Each body-normalized tissue mass divided by the supplied normalized control reference. | list |
{
"input": {
"actualIndependentlyMeasuredTissueMassesMg": [
100,
200
],
"matchedIndependentlyMeasuredBodyMassesGrams": [
10,
20
],
"callerVerifiedMatchedControlNormalizedMassMgPerGram": 5
},
"output": {
"result": {
"observedBodyNormalizedTissueMassesMgPerGram": [
10,
10
],
"observedNormalizedMassFoldChanges": [
2,
2
]
}
}
}Limitations and assumptions
Chemical identity, route, assay, exposure basis and reference applicability are caller-established. The output supplies no causal conclusion, universal toxicity threshold or authoritative regulatory determination.
Missing required fields, unknown fields, wrong types and violated rules return input issues. Numeric values and outputs must be finite. Lists and matrices must be dense, without missing cells. Numeric lists allow at most 10000 entries, subject to the narrower lengths and code rules above. Extremely large composite outputs are rejected.
Alternative or Complementary methods
For a related step, use Uterotrophic Endocrine Disruption Calculator: A uterotrophic comparison relates independently measured uterine mass to matched body mass and control context. For a complementary comparison, use Teratogenic Index Calculator: A teratogenic-index convention can compare matched maternal and developmental endpoint doses.
References
-
Hershberger Assay Calculator — tool.io
Similar or alternative tools
- Uterotrophic Endocrine Disruption Calculator
A uterotrophic comparison relates independently measured uterine mass to matched body mass and control context.
- Teratogenic Index Calculator
A teratogenic-index convention can compare matched maternal and developmental endpoint doses.