Ames Genotoxicity Calculator

Actual strain/activation/protocol-specific Amesobserved revertant fold changes. Counts, control adequacy, dose response, replicates, cytotoxicity and assay interpretation external; not diagnose genotoxicity or prescribe experimental procedures.

Description

Ames-assay revertant counts can be expressed relative to a matched control.

Ames-assay revertant counts can be expressed relative to a matched control.^[1]

Strain, activation, cytotoxicity and dose-response evidence remain necessary to interpret those fold changes.

When to use Ames Genotoxicity Calculator

Use this tool to calculate or review the supplied observations under the stated rules. Establish the chemical, exposure route, endpoint, measurement basis and independently applicable assay or assessment reference.

How to use Ames Genotoxicity Calculator and understand its results

Establish the chemical, exposure route, endpoint, measurement basis and independently applicable assay or assessment reference.
Enter actualTestedDoseCoordinates, independentlyCountedRevertantColonies, matchedControlRevertantCount, using the field meanings and rules below, then run the tool.
Read result.observedRevertantFoldChanges. Use the units and subtraction order below to interpret each value; retain negative differences where returned.
Correct any reported input issue and run again. Before using a successful result, check the stated units, signs, reference scope and observation coverage.

Input field	Meaning	Value type	Requirement
actualTestedDoseCoordinates	Actual tested dose coordinates	list of number	Required
independentlyCountedRevertantColonies	Independently counted revertant colonies	list of number	Required
matchedControlRevertantCount	Matched control revertant count	number	Required

Supply at least two strictly increasing finite dose coordinates and aligned integer observed colony counts from 0 through 10000000. Dose coordinates have no nonnegative guard. Require a positive integer matched control count through 10000000. Return each observed/control fold in order; no mutation call, significance test or dose-response fit is added.

fold_i=observedColonies_i/controlColonies

Use the matching field units and the exact boundary, ordering and reference rules above.

Output field	Meaning	Value type
result	Qualified observed calculation or explicit reference	object
result.observedRevertantFoldChanges	Individual observed colony counts divided by the matched control count.	list

json

{
  "input": {
    "actualTestedDoseCoordinates": [
      1,
      2,
      3
    ],
    "independentlyCountedRevertantColonies": [
      10,
      20,
      30
    ],
    "matchedControlRevertantCount": 10
  },
  "output": {
    "result": {
      "observedRevertantFoldChanges": [
        1,
        2,
        3
      ]
    }
  }
}

Limitations and assumptions

Chemical identity, route, assay, exposure basis and reference applicability are caller-established. The output supplies no causal conclusion, universal toxicity threshold or authoritative regulatory determination.

Missing required fields, unknown fields, wrong types and violated rules return input issues. Numeric values and outputs must be finite. Lists and matrices must be dense, without missing cells. Numeric lists allow at most 10000 entries, subject to the narrower lengths and code rules above. Extremely large composite outputs are rejected.

Alternative or Complementary methods

For a related step, use Micronucleus Frequency Calculator: Micronucleated-cell frequency counts cells classified as having micronuclei among eligible assessed cells. For a complementary comparison, use Chromosome Aberration Calculator: Aberrant-metaphase frequency counts eligible metaphases independently classified as aberrant.

References

Ames Genotoxicity Calculator — tool.io

Similar or alternative tools

Micronucleus Frequency Calculator
Micronucleated-cell frequency counts cells classified as having micronuclei among eligible assessed cells.
Chromosome Aberration Calculator
Aberrant-metaphase frequency counts eligible metaphases independently classified as aberrant.