Mitochondrial Targeting Screen

Screen N-terminal mitochondrial targeting composition; not a localization verdict.

Description

An N-terminal targeting screen compares basic and acidic residue fractions with selected criteria.

An N-terminal targeting screen compares basic and acidic residue fractions with selected criteria. Composition alone cannot establish mitochondrial import.^[1]

Screen N-terminal mitochondrial targeting composition; not a localization verdict.

When to use Mitochondrial Targeting Screen

Use this tool to screen N-terminal mitochondrial targeting composition; not a localization verdict.

Prepare the canonical sequence, peptide assignments, fitted assay parameters or upstream measurements requested. Keep corresponding entries aligned, use the stated mass and signal units, and remove FASTA headers before entering sequence text.

How to use Mitochondrial Targeting Screen and understand its results

Prepare the canonical sequence, peptide assignments, fitted assay parameters or upstream measurements requested. Keep corresponding entries aligned, use the stated mass and signal units, and remove FASTA headers before entering sequence text.
Enter sequence, nTerminalLength, minimumBasicFraction, maximumAcidicFraction using the field table and calculation rules, then run the tool.
Read basicFraction, acidicFraction, candidate. The explanation below defines the units, signs, ordering and any flags or placeholder values.
If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.

Input field	Meaning	Value type; see rules below	Requirement
sequence	Sequence	string	Required
nTerminalLength	N terminal length	number	Required
minimumBasicFraction	Minimum basic fraction	number	Required
maximumAcidicFraction	Maximum acidic fraction	number	Required

Enter a canonical sequence, positive safe-integer N-terminal length within it and finite comparison criteria. Count K/R and D/E in that fixed segment, returning both fractions and the combined inclusive flag.

The shared sequence helper trims outer whitespace and uppercases the sequence. It requires at least one residue from ACDEFGHIKLMNPQRSTVWY. Internal whitespace, FASTA headers, ambiguous letters and modified-residue notation are rejected. Sequence indexes refer to the normalized sequence; individual tools may preserve original strings in their returned lists as explained above.

candidate=(KRfraction≥minimumBasic) AND (DEfraction≤maximumAcidic)

Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.

Output field	Meaning	Value type
basicFraction	Basic fraction	number
acidicFraction	Acidic fraction	number
candidate	Candidate	boolean

json

{
  "input": {
    "sequence": "KKAA",
    "nTerminalLength": 4,
    "minimumBasicFraction": 0.4,
    "maximumAcidicFraction": 0.1
  },
  "output": {
    "basicFraction": 0.5,
    "acidicFraction": 0,
    "candidate": true
  }
}

Limitations and assumptions

Criteria are not separately limited to 0–1. Amphipathicity, cleavage sites, transport context and measured localization are not evaluated.

Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Extremely large text inputs are rejected. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors. A successfully returned false flag is an evaluated outcome; its meaning is explained above.

Alternative or Complementary methods

Use Nuclear Localization Clusters: Scan basic Lys/Arg clusters as nuclear-localization-signal candidates. For a complementary task, use Signal Peptide Core Scan: Summarize N-region charge and hydrophobic signal-peptide core.

References

Mitochondrial Targeting Screen — tool.io

Similar or alternative tools

Nuclear Localization Clusters
Scan basic Lys/Arg clusters as nuclear-localization-signal candidates.
Signal Peptide Core Scan
Summarize N-region charge and hydrophobic signal-peptide core.