Light Dark Bottle Productivity
Balance initial/light/dark bottle oxygen to net, respiration and gross production with supplied carbon stoichiometry; incubation representativeness, gas leakage and bottle effects are not corrected.
Description
Light and dark bottle incubations compare oxygen gains in light with losses in darkness.
Light and dark bottle incubations compare oxygen gains in light with losses in darkness. Their balance separates net production, respiration and gross production over the incubation period.1
The tool returns netProductivityMgOxygenPerLiterDay, respirationMgOxygenPerLiterDay, grossProductivityMgOxygenPerLiterDay, grossCarbonProductivityMgPerLiterDay. The computation uses supplied observations and parameters rather than acquiring measurements.
When to use Light Dark Bottle Productivity
Use this for the following task: Balance initial/light/dark bottle oxygen to net, respiration and gross production with supplied carbon stoichiometry; incubation representativeness, gas leakage and bottle effects are not corrected. Prepare observations and calibration parameters on the same unit and reporting basis.
How to use Light Dark Bottle Productivity and understand its results
- Prepare the measured quantities and supplied model parameters. Match the units and align paired lists before calculating.
- Enter initialOxygenMgPerLiter, lightBottleOxygenMgPerLiter, darkBottleOxygenMgPerLiter, incubationHours, carbonOxygenMolarMassRatio, photosyntheticQuotient according to the field table, then run the tool.
- Read netProductivityMgOxygenPerLiterDay, respirationMgOxygenPerLiterDay, grossProductivityMgOxygenPerLiterDay, grossCarbonProductivityMgPerLiterDay. Use the calculation below to interpret the selected mean or the model’s signs, units and comparison flags.
- Review the supplied assumptions and calibration before comparing or reporting the result.
| Input field | Meaning | Value type and bounds | Requirement |
|---|---|---|---|
| initialOxygenMgPerLiter | Initial oxygen mg per liter | number | Required |
| lightBottleOxygenMgPerLiter | Light bottle oxygen mg per liter | number | Required |
| darkBottleOxygenMgPerLiter | Dark bottle oxygen mg per liter | number | Required |
| incubationHours | Incubation hours | number | Required |
| carbonOxygenMolarMassRatio | Carbon oxygen molar mass ratio | number | Required |
| photosyntheticQuotient | Photosynthetic quotient | number | Required |
Supply nonnegative oxygen concentrations and positive incubation hours, carbon-to-oxygen molar-mass ratio and photosynthetic quotient. Normalize the measured changes to daily rates and preserve their signs.
net=(light−initial)×24/hours; respiration=(initial−dark)×24/hours; gross=net+respiration; grossCarbon=gross×massRatio/photosyntheticQuotient The symbols and boundary conditions refer to the supplied fields and the implementation described above.
| Output field | Meaning | Value type |
|---|---|---|
| netProductivityMgOxygenPerLiterDay | Net productivity mg oxygen per liter day | number |
| respirationMgOxygenPerLiterDay | Respiration mg oxygen per liter day | number |
| grossProductivityMgOxygenPerLiterDay | Gross productivity mg oxygen per liter day | number |
| grossCarbonProductivityMgPerLiterDay | Gross carbon productivity mg per liter day | number |
{
"input": {
"initialOxygenMgPerLiter": 10,
"lightBottleOxygenMgPerLiter": 12,
"darkBottleOxygenMgPerLiter": 9,
"incubationHours": 24,
"carbonOxygenMolarMassRatio": 0.375,
"photosyntheticQuotient": 1
},
"output": {
"netProductivityMgOxygenPerLiterDay": 2,
"respirationMgOxygenPerLiterDay": 1,
"grossProductivityMgOxygenPerLiterDay": 3,
"grossCarbonProductivityMgPerLiterDay": 1.125
}
}Limitations and assumptions
Daily scaling assumes the measured hourly rates persist. Bottle effects, gas leakage and differences between light and dark respiration are not corrected; this is not a whole-lake daily productivity measurement.
Numerical inputs must be finite. Input-shape and calculation checks do not establish measurement quality or the validity of a supplied calibration.
Alternative or Complementary methods
Choose Oxygen Deficit Absolute when you need its operation: Integrate oxygen deficit against supplied layer-specific saturation references and volumes; supersaturated layers contribute negative deficits, unlike a saturation-concentration model. Choose Areal Hypolimnetic Oxygen Depletion for the complementary operation: Fit net hypolimnetic oxygen-inventory depletion and normalize by area; negative depletion records replenishment, and changing hypolimnetic boundaries or advective supply require separate correction.
Background reference: The Flow of Energy from Primary Production to Higher Trophic Levels.
References
-
The Flow of Energy from Primary Production to Higher Trophic Levels — University of Michigan
Similar or alternative tools
- Oxygen Deficit Absolute
Integrate oxygen deficit against supplied layer-specific saturation references and volumes; supersaturated layers contribute negative deficits, unlike a saturation-concentration model.
- Areal Hypolimnetic Oxygen Depletion
Fit net hypolimnetic oxygen-inventory depletion and normalize by area; negative depletion records replenishment, and changing hypolimnetic boundaries or advective supply require separate correction.