Flow-Cytometry Line Coverage
Match required flow-cytometry excitation lines to available lines within a supplied tolerance.
Description
Excitation-line coverage checks each required wavelength against any available line within a tolerance.
Excitation-line coverage checks each required wavelength against any available line within a tolerance. Matching is independent for each requirement.1
Match required flow-cytometry excitation lines to available lines within a supplied tolerance.
When to use Flow-Cytometry Line Coverage
Use this tool to match required flow-cytometry excitation lines to available lines within a supplied tolerance.
Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
How to use Flow-Cytometry Line Coverage and understand its results
- Prepare the optical measurements, geometry, fitted coefficients or caller-selected thresholds requested. Confirm wavelength, radius/diameter, pulse-width, angle and unit conventions before running the calculation.
- Enter requiredLinesNanometers, availableLinesNanometers, toleranceNanometers using the field table and calculation rules, then run the tool.
- Read coveredLineCount, coveragePercent, missingLinesNanometers. The explanation below defines the units, signs, ordering and any flags or placeholder values.
- If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.
| Input field | Meaning | Value type; see rules below | Requirement |
|---|---|---|---|
| requiredLinesNanometers | Required excitation lines (nm) | list of number | Required |
| availableLinesNanometers | Available laser lines (nm) | list of number | Required |
| toleranceNanometers | Matching tolerance (nm) | number; minimum: 0 | Required |
Enter nonempty finite line lists and nonnegative nm tolerance. Return covered count, percent and missing required values in their original order. Duplicates count separately; one available line can cover multiple requirements.
covered if any |available−required|≤tolerance Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.
| Output field | Meaning | Value type |
|---|---|---|
| coveredLineCount | Covered required lines | integer; minimum: 0 |
| coveragePercent | Line coverage (%) | number; minimum: 0; maximum: 100 |
| missingLinesNanometers | Missing required lines (nm) | list of number |
{
"input": {
"requiredLinesNanometers": [
405,
488,
640
],
"availableLinesNanometers": [
404,
490,
638
],
"toleranceNanometers": 2
},
"output": {
"coveredLineCount": 3,
"coveragePercent": 100,
"missingLinesNanometers": []
}
}Limitations and assumptions
No fluorophore excitation spectrum, laser power, wavelength positivity or channel assignment is evaluated.
Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Lists are limited to 100,000 entries; the rules above may require smaller limits. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors.
Alternative or Complementary methods
Use Praseodymium Line Match: Match a requested wavelength to caller-supplied praseodymium fiber lines. For a complementary task, use He–Cd Line Selector: Select the nearer common He–Cd line to a requested wavelength.
References
-
Flow-Cytometry Line Coverage — tool.io
Similar or alternative tools
- Praseodymium Line Match
Match a requested wavelength to caller-supplied praseodymium fiber lines.
- He–Cd Line Selector
Select the nearer common He–Cd line to a requested wavelength.