Testcross 1:1 Ratio Backcross Checker
Screen ordered phenotype proportions against the named genetic ratio with caller absolute fraction tolerance; not a significance test or proof of an epistatic mechanism.
Description
Screen ordered phenotype proportions against the named genetic ratio with caller absolute fraction tolerance; not a significance test or proof of an epistatic mechanism.
A one-to-one testcross ratio can be screened against two ordered phenotype counts. The result describes proportion agreement under a caller-selected tolerance.
When to use this tool
Screen ordered phenotype proportions against the named genetic ratio with caller absolute fraction tolerance; not a significance test or proof of an epistatic mechanism.
- Prepare the supplied observedCounts, maximumAbsoluteFractionDeviation fields for this operation.
- Compare the complete example with the representation used by your own input before carrying the result into another step.
How to use the tool and how it works
- Enter observedCounts, maximumAbsoluteFractionDeviation. Use the exact text syntax, units and index conventions explained below.
- Run the tool. If input validation fails, correct the supplied fields before interpreting any result.
- Read result.expectedRatio, result.signedFractionDeviations, result.withinSuppliedTolerance, result.mechanismEstablished. Interpret false, null and empty lists using the distinctions below; they can be valid outputs.
Require 2 ordered nonnegative integer observedCounts, each at most one billion, with a positive sum. The expected ratio is 1:1. Divide each count by the observed total, subtract its expected ratio fraction and return signedFractionDeviations. Pass withinSuppliedTolerance only when every absolute deviation is at most maximumAbsoluteFractionDeviation, a number from zero through one. Return expectedRatio and mechanismEstablished false.1
| Input field | Meaning and representation | Accepted input |
|---|---|---|
| observedCounts | observed counts. | List of finite numbers; bounded by the operation |
| maximumAbsoluteFractionDeviation | maximum absolute fraction deviation. | Finite number |
| Output field | Interpretation |
|---|---|
| result | Object containing the named outputs below. |
| result.expectedRatio | The expected ratio list in the ordering and representation described above. |
| result.signedFractionDeviations | The signed fraction deviations list in the ordering and representation described above. |
| result.withinSuppliedTolerance | Whether every absolute observed-minus-expected fraction deviation is within the supplied inclusive tolerance. |
| result.mechanismEstablished | Always false: a proportion match does not establish the genetic mechanism. |
{
"input": {
"observedCounts": [
1,
1
],
"maximumAbsoluteFractionDeviation": 0
},
"output": {
"result": {
"expectedRatio": [
1,
1
],
"signedFractionDeviations": [
0,
0
],
"withinSuppliedTolerance": true,
"mechanismEstablished": false
}
}
}Limitations and assumptions
- The screen does not prove a testcross model or estimate genotype probabilities. It does not run a significance test, account for sample size uncertainty or identify the categories automatically.
- Every declared top-level field is required and unexpected fields are rejected. Numeric inputs must be finite; ordinary text fields are bounded to 8,192 UTF-16 code units, with tighter operation-specific limits where stated.
- Ordinary string lists have at most 128 entries of at most 128 code units each; numeric lists and matrices have separate bounded size checks. Nonfinite results and excessively large serialized outputs are rejected.
- Indexes are zero-based and spans have an exclusive end unless explicitly stated otherwise. A valid false, null or empty-list result is distinct from malformed input.
Alternative or Complementary methods
Keep model assumptions and phenotype-category order with each result. Ratio tolerance screens differ from goodness-of-fit inference, and sequence matches or likelihood products do not independently establish a biological mechanism or personal identity.
References
Similar or alternative tools
- Chi-Square Critical Value 0.05 df Comparison Gate Checker
Look up caller-supplied reference text by an exact query key key, retaining source, context and date without independently verifying the evidence.
- Monohybrid Cross 3:1 Phenotypic Ratio Checker
Screen ordered phenotype proportions against the named genetic ratio with caller absolute fraction tolerance; not a significance test or proof of an epistatic mechanism.