Description
Return purification fold and activity recovery.
Laboratory quantities depend on sample volume, concentration basis, blank correction, geometry and calibration. A dilution, recovery fraction or signal correction evaluates a supplied measurement model without validating the assay, identifying a substance or establishing biological or clinical performance.
Return purification fold and activity recovery.
When to use this tool
Use Purification Specific Activity Step Ratio when checking the stated laboratory bookkeeping, signal, sample or measurement convention.
How to use the tool and how it works
- Enter activityBefore, proteinBefore, activityAfter, proteinAfter. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read foldPurification, activityRecovery. Use the output convention below.
Return purification fold and activity recovery. Require positive before activity/protein and after protein, after activity >= 0; fold = (afterActivity/afterProtein)/(beforeActivity/beforeProtein), recovery = afterActivity/beforeActivity.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| activityBefore | Activity Before | Required. Finite number; strictly above 0. |
| proteinBefore | Protein Before | Required. Finite number; strictly above 0. |
| activityAfter | Activity After | Required. Finite number; minimum 0. |
| proteinAfter | Protein After | Required. Finite number; strictly above 0. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| foldPurification | Fold Purification | Required. Finite number. |
| activityRecovery | Activity Recovery | Required. Finite number. |
{
"input": {
"activityBefore": 1000,
"proteinBefore": 100,
"activityAfter": 800,
"proteinAfter": 20
},
"output": {
"foldPurification": 4,
"activityRecovery": 0.8
}
}Limitations and assumptions
- Protein and activity units must match across steps; values above one are allowed and actual purity or assay validity is not established.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use verified sample records, calibrated equipment and an appropriate validated measurement method; assess uncertainty and practical laboratory conditions independently.
References
Similar or alternative tools
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