Description
Return background-adjusted spike recovery.
Laboratory quantities depend on sample volume, concentration basis, blank correction, geometry and calibration. A dilution, recovery fraction or signal correction evaluates a supplied measurement model without validating the assay, identifying a substance or establishing biological or clinical performance.
Return background-adjusted spike recovery.
When to use this tool
Use Assay Spike Recovery Background Term when checking the stated laboratory bookkeeping, signal, sample or measurement convention.
How to use the tool and how it works
- Enter spiked, unspiked, spikeAdded. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read recoveryFraction. Use the output convention below.
Return background-adjusted spike recovery. Require spikeAdded > 0; recoveryFraction = (spiked-unspiked)/spikeAdded.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| spiked | Spiked | Required. Finite number. |
| unspiked | Unspiked | Required. Finite number. |
| spikeAdded | Spike Added | Required. Finite number; strictly above 0. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| recoveryFraction | Recovery Fraction | Required. Finite number. |
{
"input": {
"spiked": 15,
"unspiked": 5,
"spikeAdded": 8
},
"output": {
"recoveryFraction": 1.25
}
}Limitations and assumptions
- The result can be negative or above one; comparable units, linear response, matrix behavior and assay validity are not verified.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use verified sample records, calibrated equipment and an appropriate validated measurement method; assess uncertainty and practical laboratory conditions independently.
References
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