Use Band Intensity Ratio when you need to normalise a western blot target band by dividing its densitometry intensity by the loading control intensity of the same lane. Enter Target band intensity and Loading control intensity using the definitions and units below so the result can be reproduced.

Target band intensity

Densitometry intensity of the target protein band; must not be negative.

Loading control intensity

Densitometry intensity of the loading control band; must be greater than zero.

The published tool contract uses this rule: Normalise a western blot target band by dividing its densitometry intensity by the loading control intensity of the same lane.

Normalized ratio

Target band intensity normalised to the loading control.

A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.

Retain the original Target band intensity and Loading control intensity values and their units alongside the result. Two-Band EVI is useful when you instead need to compute the two-band Enhanced Vegetation Index as 2.5 * (NIR - red) / (NIR + 2.4 * red + 1) from reflectances between zero and one, rejecting degenerate reflectances that zero the denominator. APTT Ratio provides another view by helping you compute the activated partial thromboplastin time ratio as the patient APTT divided by the control APTT.

• Two-Band EVI

  Compute the two-band Enhanced Vegetation Index as 2.5 * (NIR - red) / (NIR + 2.4 * red + 1) from reflectances between zero and one, rejecting degenerate reflectances that zero the denominator.

• APTT Ratio

  Compute the activated partial thromboplastin time ratio as the patient APTT divided by the control APTT.

• Gibbs-Donnan Ratio

  Compute the Gibbs-Donnan ratio as the concentration of permeant ion inside a cell divided by its concentration outside.