Use Serial Dilution Biology when you need to compute the endpoint concentration of a serial dilution: finalConcentration = startConcentration x transferRatio^steps, with a strictly fractional transfer ratio and at least one step. Enter Start concentration, Transfer ratio, and Dilution steps using the definitions and units below so the result can be reproduced.

Start concentration

Starting concentration; must be greater than zero.

Transfer ratio

Transfer ratio per step, strictly between 0 and 1.

Dilution steps

Number of dilution steps; integer of at least 1.

The published tool contract uses this rule: Compute the endpoint concentration of a serial dilution: finalConcentration = startConcentration x transferRatio^steps, with a strictly fractional transfer ratio and at least one step.

Final concentration

Final concentration after all steps.

A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.

Retain the original Start concentration, Transfer ratio, and Dilution steps values and their units alongside the result. Lab Dilution Series Step is useful when you instead need to compute one serial dilution step: dilutedConcentration = previousConcentration x transferRatio, where the transfer ratio lies strictly between 0 and 1 and defaults to 0.5 for a two-fold dilution. Cell Concentration From Dilution provides another view by helping you recover the original cell concentration by scaling a counted dilution.

• Lab Dilution Series Step

  Compute one serial dilution step: dilutedConcentration = previousConcentration x transferRatio, where the transfer ratio lies strictly between 0 and 1 and defaults to 0.5 for a two-fold dilution.

• Cell Concentration From Dilution

  Recover the original cell concentration by scaling a counted dilution.

• Cell Dilution C1V1=C2V2

  Solve C1V1=C2V2 for the stock volume needed to reach a target concentration.