Lab Dilution Series Step
Compute one serial dilution step: dilutedConcentration = previousConcentration x transferRatio, where the transfer ratio lies strictly between 0 and 1 and defaults to 0.5 for a two-fold dilution.
Description
Compute one serial dilution step: dilutedConcentration = previousConcentration x transferRatio, where the transfer ratio lies strictly between 0 and 1 and defaults to 0.5 for a two-fold dilution.
Lab Dilution Series Step is a focused biology tool that compute one serial dilution step: dilutedConcentration = previousConcentration x transferRatio, where the transfer ratio lies strictly between 0 and 1 and defaults to 0.5 for a two-fold dilution. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use Lab Dilution Series Step
Use Lab Dilution Series Step when you need to compute one serial dilution step: dilutedConcentration = previousConcentration x transferRatio, where the transfer ratio lies strictly between 0 and 1 and defaults to 0.5 for a two-fold dilution. Enter Previous concentration and Transfer ratio using the definitions and units below so the result can be reproduced.
- Previous concentration
- Concentration entering the step; must be greater than zero.
- Transfer ratio
- Fraction transferred into the next tube, strictly between 0 and 1; defaults to 0.5.
How Lab Dilution Series Step calculates the result
The published tool contract uses this rule: Compute one serial dilution step: dilutedConcentration = previousConcentration x transferRatio, where the transfer ratio lies strictly between 0 and 1 and defaults to 0.5 for a two-fold dilution.
- Diluted concentration
- Concentration after the single dilution step.
Limitations of Lab Dilution Series Step
A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.
Alternative or Complementary measurements
Retain the original Previous concentration and Transfer ratio values and their units alongside the result. Serial Dilution Biology is useful when you instead need to compute the endpoint concentration of a serial dilution: finalConcentration = startConcentration x transferRatio^steps, with a strictly fractional transfer ratio and at least one step. Leakage Two Step provides another view by helping you compute the electrolyte leakage ratio as the initial conductivity divided by the maximum conductivity measured after a second boil; the result is a fraction between zero and one.
References
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Serial dilution — Wikipedia contributors
Similar or alternative tools
- Serial Dilution Biology
Compute the endpoint concentration of a serial dilution: finalConcentration = startConcentration x transferRatio^steps, with a strictly fractional transfer ratio and at least one step.
- Leakage Two Step
Compute the electrolyte leakage ratio as the initial conductivity divided by the maximum conductivity measured after a second boil; the result is a fraction between zero and one.
- CFSE Dilution Factor
Estimate cell division count from CFSE fluorescence dilution using the base two logarithm of the initial over current intensity.