Reverse Complement
Build the reverse complement of a DNA sequence by complementing bases then reversing.
Description
Build the reverse complement of a DNA sequence by complementing bases then reversing.
Reverse Complement is a focused biology tool that build the reverse complement of a DNA sequence by complementing bases then reversing. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use Reverse Complement
Use Reverse Complement when you need to build the reverse complement of a DNA sequence by complementing bases then reversing. Enter Sequence using the definitions and units below so the result can be reproduced.
- Sequence
- A sequence, category, or text value.
How Reverse Complement calculates the result
The published tool contract uses this rule: Build the reverse complement of a DNA sequence by complementing bases then reversing.
- Reverse complement strand
- The calculated reverse complement strand.
Limitations of Reverse Complement
The calculation does not propagate measurement uncertainty, sampling error, or biological and chemical variability.
Alternative or Complementary measurements
Retain the original Sequence values and their units alongside the result. DNA Complement is useful when you instead need to map each DNA base to its complement, pairing A with T and G with C. Salt-Adjusted Tm provides another view by helping you estimate DNA melting temperature with a monovalent salt correction from GC fraction and sequence length.
References
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Complementarity (molecular biology) — Wikipedia contributors
Similar or alternative tools
- DNA Complement
Map each DNA base to its complement, pairing A with T and G with C.
- Salt-Adjusted Tm
Estimate DNA melting temperature with a monovalent salt correction from GC fraction and sequence length.
- Transcribe To RNA
Transcribe a DNA sequence to RNA by replacing every thymine with uracil.