Use Relative Expression Fold when you need to convert a delta delta CT value into a relative fold change using the 2^-ddCt method. Enter Delta delta CT using the definitions and units below so the result can be reproduced.

Delta delta CT

A finite measured or assumed value, expressed in cycles.

The published tool contract uses this rule: Convert a delta delta CT value into a relative fold change using the 2^-ddCt method.

Relative fold change

The calculated relative fold change in fold.

The calculation does not propagate measurement uncertainty, sampling error, or biological and chemical variability.

Retain the original Delta delta CT values and their units alongside the result. Fold Change is useful when you instead need to compute the fold change between condition and control means with its log2 value. Relative Fitness provides another view by helping you compute relative fitness w = survived offspring / maximum survived offspring in the population.

• Fold Change

  Compute the fold change between condition and control means with its log2 value.

• Relative Fitness

  Compute relative fitness w = survived offspring / maximum survived offspring in the population.

• Relative Refractory Period

  Compute the fraction of relative refractory recovery clamped to 0..1 from elapsed time, the absolute refractory duration, and the relative recovery window.