Delta Delta CT
Calculate the delta delta CT value by subtracting the control delta CT from the treated delta CT.
Description
Calculate the delta delta CT value by subtracting the control delta CT from the treated delta CT.
Delta Delta CT is a focused biology tool that calculate the delta delta CT value by subtracting the control delta CT from the treated delta CT. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use Delta Delta CT
Use Delta Delta CT when you need to calculate the delta delta CT value by subtracting the control delta CT from the treated delta CT. Enter Treated delta CT and Control delta CT using the definitions and units below so the result can be reproduced.
- Treated delta CT
- A finite measured or assumed value, expressed in cycles.
- Control delta CT
- A finite measured or assumed value, expressed in cycles.
How Delta Delta CT calculates the result
The published tool contract uses this rule: Calculate the delta delta CT value by subtracting the control delta CT from the treated delta CT.1
- Delta delta CT
- The calculated delta delta ct in cycles.
Limitations of Delta Delta CT
The calculation does not propagate measurement uncertainty, sampling error, or biological and chemical variability.
Alternative or Complementary measurements
Retain the original Treated delta CT and Control delta CT values and their units alongside the result. Delta CT is useful when you instead need to calculate the signed delta CT value by subtracting the reference Ct from the target Ct. qPCR Copy Number provides another view by helping you convert a double-stranded DNA mass in nanograms to molecule count using length and the mean base pair mass of 650 daltons.
References
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Real-time polymerase chain reaction — Wikipedia contributors
Similar or alternative tools
- Delta CT
Calculate the signed delta CT value by subtracting the reference Ct from the target Ct.
- qPCR Copy Number
Convert a double-stranded DNA mass in nanograms to molecule count using length and the mean base pair mass of 650 daltons.
- Relative Expression Fold
Convert a delta delta CT value into a relative fold change using the 2^-ddCt method.