Plasmid Copy Number
Estimate plasmid copies per genome as the concentration ratio scaled by the inverse ratio of molecule lengths.
Description
Estimate plasmid copies per genome as the concentration ratio scaled by the inverse ratio of molecule lengths.
Plasmid Copy Number is a focused biology tool that estimate plasmid copies per genome as the concentration ratio scaled by the inverse ratio of molecule lengths. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use Plasmid Copy Number
Use Plasmid Copy Number when you need to estimate plasmid copies per genome as the concentration ratio scaled by the inverse ratio of molecule lengths. Enter Plasmid concentration, Genome concentration, Plasmid length, and Genome size using the definitions and units below so the result can be reproduced.
- Plasmid concentration
- A finite measured or assumed value, expressed in ng/µL.
- Genome concentration
- A finite measured or assumed value, expressed in ng/µL.
- Plasmid length
- A finite measured or assumed value, expressed in bp.
- Genome size
- A finite measured or assumed value, expressed in bp.
How Plasmid Copy Number calculates the result
The published tool contract uses this rule: Estimate plasmid copies per genome as the concentration ratio scaled by the inverse ratio of molecule lengths.1
- Plasmid copies per genome
- The calculated plasmid copies per genome.
Limitations of Plasmid Copy Number
The output is an estimate tied to the stated model and coefficients; it is not a direct measurement and should retain that label when reported.
Alternative or Complementary measurements
Retain the original Plasmid concentration, Genome concentration, Plasmid length, and Genome size values and their units alongside the result. Plasmid Copies Per Cell is useful when you instead need to estimate plasmid copies per cell from plasmid and genomic DNA masses, correcting the mass ratio for their different molecule lengths. qPCR Copy Number provides another view by helping you convert a double-stranded DNA mass in nanograms to molecule count using length and the mean base pair mass of 650 daltons.
References
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Plasmid copy number — Wikipedia contributors
Similar or alternative tools
- Plasmid Copies Per Cell
Estimate plasmid copies per cell from plasmid and genomic DNA masses, correcting the mass ratio for their different molecule lengths.
- qPCR Copy Number
Convert a double-stranded DNA mass in nanograms to molecule count using length and the mean base pair mass of 650 daltons.
- DNA Copy Number
Calculate the number of DNA molecules from mass in nanograms and length in base pairs.