Reverse Strand One Coordinate Transform Tool

Description

Reverse one zero-based sequence coordinate.

Genome annotations depend on coordinate origin, endpoint inclusion, strand and reading-phase conventions. A local offset or phase token can be handled precisely while a complete transcript, reference identity and biological reading frame remain separate validation questions.

Reverse one zero-based sequence coordinate.

When to use this tool

Use Reverse Strand One Coordinate Transform when checking the stated genomic coordinate, annotation phase or attribute-value convention.

How to use the tool and how it works

Enter position, sequenceLength. Use the units and conventions below.
Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
Read reversePosition. Use the output convention below.

Reverse one zero-based sequence coordinate. Require positive whole sequenceLength and position in [0,length); reversePosition = length-1-position.^[1]

Input field	Meaning and units	Accepted input
position	Position	Required. Safe integer; minimum 0.
sequenceLength	Sequence Length	Required. Safe integer; minimum 1.

Output field	Interpretation	Returned shape
reversePosition	Reverse Position	Required. Safe integer.

json

{
  "input": {
    "position": 2,
    "sequenceLength": 10
  },
  "output": {
    "reversePosition": 7
  }
}

Limitations and assumptions

Only one coordinate is transformed; intervals, strand annotations, sequence content and reference identity are not validated.
Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.

Alternative or Complementary methods

Use the intended complete BED/GFF/GTF format, reference assembly and transcript model to validate full annotations and biological meaning.

References

Calculation and input contract: bioinformatics/reverse-strand-one-coordinate-transform — tool.io

Similar or alternative tools

Phasing Block Span
Return a phasing-block span under an endpoint convention.
Genomic Left Closed Right Open Length Term
Return length and last included position of a half-open interval.