Description
Decode one FASTQ quality character under a supplied offset.
Sequence text, ambiguity codes and encoded quality scores describe different information. Literal alphabets and Phred transformations can be checked without identifying a molecule or proving calibrated sequencing accuracy; missingness and reporting encodings require explicit conventions.
Decode one FASTQ quality character under a supplied offset.
When to use this tool
Use Bioinformatics: Fastq Quality Char Phred Score when checking the stated sequence token, ambiguity, quality symbol or local trimming convention.
How to use the tool and how it works
- Enter character, offset. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read result. Expand result to inspect the returned fields.
Decode one FASTQ quality character under a supplied offset. Require offset 33 or 64 and one character with code between offset and 126; phredScore = code-offset.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| character | Character | Required. Text. Implementation limit: 8192 Unicode code points and 16384 UTF-16 code units. |
| offset | Explicit ASCII quality offset, 33 or 64; not detected from data. | Required. Safe integer. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| result | Returned calculated value or nested object under the stated output convention. | Required. Object. |
| result.phredScore | Phred Score | Calculated result member: number. |
{
"input": {
"character": "I",
"offset": 33
},
"output": {
"result": {
"phredScore": 40
}
}
}Limitations and assumptions
- Offset is not detected and dialect compatibility is not verified; one character is decoded without assessing read quality calibration.
- Outputs are nested inside result. Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use a complete format-aware sequence/quality workflow and the appropriate reference/genetic code; assess biological identity and quality calibration independently.
References
Similar or alternative tools
- FASTQ Quality Trimming One End Budget
Trim a consecutive low-quality run from one supplied end.
- Pileup Readstart Quality Symbol
Decode a pileup read-start mapping-quality symbol.