Description
Return a cleavage-count peptide budget.
Peptide mass and cleavage budgets require explicit assumptions about chain structure, bond formation, released species and mass convention. Adding or subtracting supplied contributions does not determine a sequence, reaction mechanism or the actual products of digestion or cyclization.
Return a cleavage-count peptide budget.
When to use this tool
Use Protein Digest Peptide Count Cleavage Budget when checking the stated peptide cleavage or mass-adjustment convention.
How to use the tool and how it works
- Enter cleavableBonds, missedCleavages, chains. Use the units and conventions below.
- Run the tool. Correct any format, range, or cross-field input error before interpreting the output.
- Read realizedCleavages, peptideCount. Use the output convention below.
Return a cleavage-count peptide budget. Require nonnegative whole cleavableBonds, missedCleavages within it and chains >= 1; realizedCleavages = cleavable-missed, peptideCount = chains+realized.1
| Input field | Meaning and units | Accepted input |
|---|---|---|
| cleavableBonds | Cleavable Bonds | Required. Safe integer; minimum 0. |
| missedCleavages | Missed Cleavages | Required. Safe integer; minimum 0. |
| chains | Chains | Required. Safe integer; minimum 1. |
| Output field | Interpretation | Returned shape |
|---|---|---|
| realizedCleavages | Realized Cleavages | Required. Safe integer. |
| peptideCount | Peptide Count | Required. Safe integer. |
{
"input": {
"cleavableBonds": 10,
"missedCleavages": 2,
"chains": 1
},
"output": {
"realizedCleavages": 8,
"peptideCount": 9
}
}Limitations and assumptions
- This assumes each counted cleavage separates a linear chain; sequence specificity, crosslinks and actual digestion products are not inferred.
- Inputs must satisfy both the field types and the cross-field conditions above. Invalid input causes an error; a successful false flag or documented placeholder is a different result.
- Numeric calculations use binary floating point unless exact decimal-integer arithmetic is explicitly stated. No hidden measurement tolerance or decimal rounding is added; nonfinite or out-of-contract results are rejected. Numeric signed-zero results are exported as zero.
Alternative or Complementary methods
Use the complete sequence, verified chemistry and a consistent monoisotopic/average mass convention for actual molecular or digestion-product analysis.
References
Similar or alternative tools
- Peptide Disulfide Mass Adjustment
Return mass change for supplied disulfide bonds.
- Peptide Cyclization Mass Delta
Return cyclization/adduct mass bookkeeping.