Biofilm Eradication Endpoint

Find the lowest observed biofilm exposure concentration meeting a supplied post-exposure recovery criterion; units and protocol must be consistent.

Description

A biofilm exposure endpoint depends on recoverable organisms after treatment under a supplied recovery criterion.

A biofilm exposure endpoint depends on recoverable organisms after treatment under a supplied recovery criterion. It differs from merely observing inhibited growth during exposure.^[1]

Find the lowest observed biofilm exposure concentration meeting a supplied post-exposure recovery criterion; units and protocol must be consistent.

When to use Biofilm Eradication Endpoint

Use this tool to find the lowest observed biofilm exposure concentration meeting a supplied post-exposure recovery criterion; units and protocol must be consistent.

Prepare the sample, count, growth or assay measurements requested by this tool. Confirm units, sampling periods and reporting bases; supply independently established comparison criteria where requested.

How to use Biofilm Eradication Endpoint and understand its results

Prepare the sample, count, growth or assay measurements requested by this tool. Confirm units, sampling periods and reporting bases; supply independently established comparison criteria where requested.
Enter concentrations, recoveryCfu, maximumAllowedRecoveryCfu using the field table and calculation rules, then run the tool.
Read mbecConcentration, endpointFound, rightCensored. The explanation below defines the units, signs, ordering and any flags or placeholder values.
If the tool reports an input issue, correct the values and run it again. Apply the stated assumptions when interpreting a successfully returned result.

Input field	Meaning	Value type; see rules below	Requirement
concentrations	Concentrations	list of number	Required
recoveryCfu	Recovery cfu	list of number	Required
maximumAllowedRecoveryCfu	Maximum allowed recovery cfu	integer; minimum: 0	Required

Enter aligned nonempty increasing positive concentrations and nonincreasing nonnegative integer recovery counts, plus a nonnegative integer allowed recovery. The tool returns the first qualifying concentration and endpointFound. If none qualifies, mbecConcentration is the zero placeholder and rightCensored is true.

endpoint=first increasing concentration with recoveryCFU≤allowance

Units, indexing, boundary conditions and exceptional cases are defined in the calculation rules above.

Output field	Meaning	Value type
mbecConcentration	Mbec concentration	number
endpointFound	Endpoint found	boolean
rightCensored	Right censored	boolean

json

{
  "input": {
    "concentrations": [
      1,
      2,
      4
    ],
    "recoveryCfu": [
      100,
      10,
      0
    ],
    "maximumAllowedRecoveryCfu": 0
  },
  "output": {
    "mbecConcentration": 4,
    "endpointFound": true,
    "rightCensored": false
  }
}

Limitations and assumptions

No interpolation, universal eradication criterion or protocol validation is supplied. Zero recovery can reflect a detection limit; the result does not establish clinical eradication or treatment effectiveness.

Numeric inputs must be finite. Follow the calculation-specific rules for overflow, underflow and exceptional cases. Lists are limited to 100,000 entries; the rules above may require smaller limits. Missing required fields, extra fields, wrong value types and violations of the calculation rules are input errors. A successfully returned false flag is an evaluated outcome; its meaning is explained above.

Alternative or Complementary methods

Use Bactericidal Survival Endpoint: Find the first dilution meeting a caller-defined bactericidal kill fraction based on survivor counts, rather than merely recording MBC. For a complementary task, use Growth Inhibition MIC Endpoint: Derive the observed MIC from a monotone dilution-growth series, preserving below- and above-range censoring.

References

Biofilm Eradication Endpoint — tool.io

Similar or alternative tools

Bactericidal Survival Endpoint
Find the first dilution meeting a caller-defined bactericidal kill fraction based on survivor counts, rather than merely recording MBC.
Growth Inhibition MIC Endpoint
Derive the observed MIC from a monotone dilution-growth series, preserving below- and above-range censoring.