Use Calibration Slope when you need to compute the calibration curve slope from two concentration and response pairs. Enter Response high, Response low, Concentration high, and Concentration low using the definitions and units below so the result can be reproduced.

Response high

A finite measured or assumed value.

Response low

A finite measured or assumed value.

Concentration high

A finite measured or assumed value, expressed in mol/L.

Concentration low

A finite measured or assumed value, expressed in mol/L.

The published tool contract uses this rule: Compute the calibration curve slope from two concentration and response pairs.

Calibration slope

The calculated calibration slope.

A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.

Retain the original Response high, Response low, Concentration high, and Concentration low values and their units alongside the result. Calibration Intercept is useful when you instead need to compute the calibration curve intercept from the low calibration point and the slope. Analytical Detection Limit provides another view by helping you estimate a detection limit from response standard deviation and calibration slope using the stated 3.3σ/slope convention. This is one common calibration-based estimate, not a universal regulatory limit.

• Calibration Intercept

  Compute the calibration curve intercept from the low calibration point and the slope.

• Analytical Detection Limit

  Estimate a detection limit from response standard deviation and calibration slope using the stated 3.3σ/slope convention. This is one common calibration-based estimate, not a universal regulatory limit.

• Analytical Quantification Limit

  Estimate a quantification limit from response standard deviation and calibration slope using the stated 10σ/slope convention. Confirm the method-specific definition before reporting an official LOQ.