OD600 To CFU
Convert an OD600 absorbance reading to an estimated bacterial density: CFU/mL = OD600 x conversionFactor, using a species-dependent factor that defaults to 1e9 CFU per OD600 unit.
Description
Convert an OD600 absorbance reading to an estimated bacterial density: CFU/mL = OD600 x conversionFactor, using a species-dependent factor that defaults to 1e9 CFU per OD600 unit.
OD600 To CFU is a focused biology tool that convert an OD600 absorbance reading to an estimated bacterial density: CFU/mL = OD600 x conversionFactor, using a species-dependent factor that defaults to 1e9 CFU per OD600 unit. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use OD600 To CFU
Use OD600 To CFU when you need to convert an OD600 absorbance reading to an estimated bacterial density: CFU/mL = OD600 x conversionFactor, using a species-dependent factor that defaults to 1e9 CFU per OD600 unit. Enter OD600 and Conversion factor using the definitions and units below so the result can be reproduced.
- OD600
- Optical density at 600 nm; must not be negative.
- Conversion factor
- Calibration factor in CFU per OD600 unit; defaults to 1e9.
How OD600 To CFU calculates the result
The published tool contract uses this rule: Convert an OD600 absorbance reading to an estimated bacterial density: CFU/mL = OD600 x conversionFactor, using a species-dependent factor that defaults to 1e9 CFU per OD600 unit.1
- Estimated CFU/mL
- Estimated viable cell density in CFU per mL.
Limitations of OD600 To CFU
The output is an estimate tied to the stated model and coefficients; it is not a direct measurement and should retain that label when reported.
Alternative or Complementary measurements
Retain the original OD600 and Conversion factor values and their units alongside the result. CFU Per mL is useful when you instead need to estimate colony-forming units per milliliter from a plate colony count, dilution factor, and plating volume. CFU Total Estimate provides another view by helping you estimate the total colony-forming units in a culture from concentration and volume.
References
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OD600 — Wikipedia contributors
Similar or alternative tools
- CFU Per mL
Estimate colony-forming units per milliliter from a plate colony count, dilution factor, and plating volume.
- CFU Total Estimate
Estimate the total colony-forming units in a culture from concentration and volume.
- Hill Number N1
Convert a natural-log Shannon entropy to the Hill number of order one, the effective number of equally common species, as exp(H).