Use Phase Mitotic Fraction when you need to compute the fraction of cells positive for a specific mitotic phase marker. Enter Phase-positive cells and Total cells using the definitions and units below so the result can be reproduced.

Phase-positive cells

An integer count or setting greater than or equal to 0.

Total cells

An integer count or setting greater than or equal to 0.

The published tool contract uses this rule: Compute the fraction of cells positive for a specific mitotic phase marker.^[1]

Mitotic phase fraction

The calculated mitotic phase fraction.

A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.

Retain the original Phase-positive cells and Total cells values and their units alongside the result. S Phase Fraction is useful when you instead need to compute the fraction of cells in S phase relative to the total counted cell population. Mitotic Index % provides another view by helping you calculate the mitotic index as the percentage of counted cells in mitosis.

• S Phase Fraction

  Compute the fraction of cells in S phase relative to the total counted cell population.

• Mitotic Index %

  Calculate the mitotic index as the percentage of counted cells in mitosis.

• M Phase Percentage

  Compute the mitotic fraction of a cell population as a percentage of cells in M phase.