Hill EC50 Fit Check
Check a Hill-Langmuir fit by predicting the effect fraction at a concentration from the fitted EC50 and hill slope.
Description
Check a Hill-Langmuir fit by predicting the effect fraction at a concentration from the fitted EC50 and hill slope.
Hill EC50 Fit Check is a focused biology tool that check a Hill-Langmuir fit by predicting the effect fraction at a concentration from the fitted EC50 and hill slope. It calculates locally from the values you enter and exposes the result without changing the source observations.1
When to use Hill EC50 Fit Check
Use Hill EC50 Fit Check when you need to check a Hill-Langmuir fit by predicting the effect fraction at a concentration from the fitted EC50 and hill slope. Enter Concentration, EC50, and Hill slope using the definitions and units below so the result can be reproduced.
- Concentration
- Ligand concentration; must not be negative.
- EC50
- Half-maximal effective concentration from the fit; must be greater than zero.
- Hill slope
- Hill slope of the curve; must be greater than zero.
How Hill EC50 Fit Check calculates the result
The published tool contract uses this rule: Check a Hill-Langmuir fit by predicting the effect fraction at a concentration from the fitted EC50 and hill slope.
- Predicted effect fraction
- Predicted response as a fraction between zero and one.
Limitations of Hill EC50 Fit Check
A ratio or percentage can conceal the scale of its numerator and denominator and becomes sensitive to measurement error when the denominator is small.
Alternative or Complementary measurements
Retain the original Concentration, EC50, and Hill slope values and their units alongside the result. EC50 From Hill is useful when you instead need to estimate the half-maximal effective concentration by inverting the Hill equation. Hill Number N2 provides another view by helping you convert a Simpson concentration to the Hill number of order two as its reciprocal.
References
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EC50 — Wikipedia contributors
Similar or alternative tools
- EC50 From Hill
Estimate the half-maximal effective concentration by inverting the Hill equation.
- Hill Number N2
Convert a Simpson concentration to the Hill number of order two as its reciprocal.
- Hill Number N0
Return the Hill number of order zero, which equals species richness and therefore passes the species count through unchanged.