Use this calculation for cell-culture or microscopy observations collected with one counting rule, assay gate, calibration, and unit convention. It is suited to documenting dilution, growth, labeling, geometry, or population fractions.

Migration distance

A finite measured or assumed value greater than or equal to 0, expressed in um.

Hours elapsed

A finite measured or assumed value.

The implementation follows this calculation rule: Compute cell migration speed as distance traveled divided by hours elapsed.

Migration speed

The calculated migration speed in um/hour.

Counts and fluorescence measurements can be biased by clumping, debris, segmentation, gating, background signal, saturation, sampling fields, or unequal viability. A calculated percentage does not identify the biological mechanism behind a change. Ratios and percentages become unstable when their denominator is small, uncertain, or defined differently between samples.

Retain representative images or cytometry plots and include biological replicates. Orthogonal viability, proliferation, apoptosis, or direct counting assays can distinguish effects that a single cell metric conflates.

• Specific Growth Rate

  Calculate the specific growth rate in inverse hours from two counts.

• Confluence %

  Compute the percentage of a culture vessel surface covered by cells from occupied and total flask areas.

• Cell Viability %

  Calculate the viability percentage from viable and total cell counts.